[22] In mice, increased miR-155 expression was also induced by stimulation with lipopolysaccharides (LPS), suggesting that

miR-155 promotes the production of tumor necrosis factor-α (TNF-α).[25] Similarly, in human monocyte-derived dendritic cells, the expression of miR-155 by LPS stimulation was the most strongly induced of all miRNAs tested, suggesting that miR-155 increases the production of proinflammatory cytokines by regulating the TLR/interleukin-1 (IL-1) signaling pathways.[26] Future studies should investigate the relationship between miR-155 expression and the serum levels of proinflammatory cytokines, such as TNF-α and IL-1. In PBC, a significant increase in miR-146a expression was also observed. MiR-146 is a negative regulator of TLRs that reduces inflammatory mediators, such as IL-1 and IL-8, in response to TLR stimulation.[27, 28] Among CHIR-99021 cost miRNAs, miR-146 and

miR-155 are considered to play particularly important roles in innate immune response.[10] In the present study, both miR-146a and miR-155 exhibited increased expression in PBMCs of PBC patients. Combined with the expression pattern of miRNAs in PBC, this finding suggests the involvement of TLR-mediated immune response in PBC. A significant increase in miR-299-5p expression was observed in the PBC patients included in this study compared to AIH patients. In particular, the expression of miR-299-5p in PBC patients resistant to treatment was significantly increased compared to that Z-VAD-FMK purchase in healthy controls. A previous study has also MCE公司 documented increased expression of miR-299-5p in the liver tissue of PBC patients.[14] In the evaluation

of the relationship between miR-299-5p expression and clinical test data, miR-299-5p expression was significantly and positively correlated with ALP, GGT, TB and IgM levels, clinical markers characteristic of PBC, suggesting that unknown proteins targeted by miR-299-5p are associated with the disease activity and condition of PBC. In addition, the miR-299-5p expression level was significantly higher in PBC patients with a CA of 2–3 than in those with a CA of 0–1. Previous studies have also shown that a response to UDCA treatment is not related to interface hepatitis, but is more closely related to ductopenia.[29] On the other hand, miR-299-5p expression in patients with PBC-AIH overlap syndrome, a proposed subtype of PBC, was more similar to that in AIH patients than to that in PBC patients, suggesting that PBC-AIH overlap syndrome is more similar to AIH than to PBC in terms of the expression pattern of miRNAs. In the PBMCs of PBC patients, a significant increase in miR-328 expression was also observed compared to AIH. A significantly increased expression of miR-328 in the liver tissue of PBC patients has also been reported in a previous study.