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supplement for an 8-week period prior to the start of the study; and e) agreed not to ingest any other nutritional supplements, or non-prescription drugs that might affect the study BV-6 cell line parameters. Prior to enrolling in the study, subjects were informed of the experimental procedures as well as the potential risks and benefits associated
with the study; however, subjects were not informed of the study’s purpose. To be included in the study, each subject provided written consent in accordance with the Declaration of Helsinki. The study was approved by the research ethics committee of the Faculty of Medicine of the University of Sfax, Sfax, Tunisia. Experimental design Ramadan began on August 1 and ended on August 30, 2011. The average duration of the fast was approximately 15 h. The study was conducted in Tunisia, where daytime temperatures were 34 ± 1°C and relative humidity was 57 ± 4%. Subjects visited the laboratory on two separate occasions: two days before Ramadan (Bef-R) and on the 29th day of Ramadan (End-R). In the morning of each visit (approximately 10:30 a.m.), they underwent anthropometric measurements, completed a dietary questionnaire, and provided fasting blood and urine samples. They were instructed not to consume any food or energy-containing beverage after 11:00 p.m. on the day before each visit. Because of the time of sunset, this meant that the fasting subjects had only four hours (between 7:00 and 11:00 p.m.) on the evening before the test at End-R in which to consume food and fluid. Seventeen days before the beginning of Ramadan, subjects underwent
a test of 10 repetitions maximum (10 RM) for the following exercises: bench press, barbell squat, biceps curl, lying triceps Baricitinib curl, seated shoulder press behind the neck and barbell row. During the 10 RM testing, the mass of all weight plates and bars that were used was determined with a precision scale. The actual mass of all plates and bars was then used to calculate the 10 RM of each exercise. During the 10 RM tests, each subject had a maximum of 5 attempts on each exercise with 2- to 5-minute intervals between attempts. After each attempt, subjects add or remove weight as required. After the 10 RM load in a specific exercise was determined, an interval no shorter than 10 minutes was allowed before the 10 RM determination of the next exercise.