For example, members of the family Flavobacteriaceae can colonize diverse ecological niches with a wide range of physical-chemical characteristics [25]. It is also possible that our classification is too broad, even at subtype level, to capture the possible patterns of environmental specificity. To exclude possible biases due to unequal size of the samples, we created subsets comprising just samples of comparable size. The results of cosmopolitanism and ubiquity for two of these datasets are shown in Additional file 2, Figure S1, showing that the general trends exposed above are well conserved in these and other

subsets. Cosmopolitanism and specificity patterns can also be LCZ696 solubility dmso revealed by inspecting the evenness of the distribution of a particular taxon in the different environments. This can be done by calculating MK5108 biodiversity indices. For a particular taxon, high diversity values indicate both presence in more environments and a well-balanced distribution across them, as expected for ubiquitous families, while low diversity indicates preference for some environment(s). The results (Additional file 3, Table S2) suggest that the most diverse families with respect to their environmental distribution are Pseudomonadaceae, Comamonadaceae, Caulobacteraceae, Flavobacteriaceae and Xanthomonadaceae, while amongst

the least diverse families we find Pyrodictiaceae, Aquificaceae and Nautiliaceae (in hydrothermal environments), Dynein Thermoactinomycetaceae (soil), Sulfolobaceae (geothermal), Oscillospiraceae and Lachnospiraceae (gut). It is apparent, however, that even in the absence of total specificity, some taxa show a marked preference for some environments. For instance, some archaeal clades have been found mostly, but not exclusively, in thermal samples. To quantify these

preferences (affinities), we used a Bayesian hierarchical statistical model for detecting differences between the observed and expected distributions of abundances of the taxa in the environments, under the assumption of statistical independence between taxa and environments. The results are presented in Additional file 4, Figure S2. The highest affinities were found for taxa present in thermal environments (families Aquificaceae, Sulfolobaceae, Thermoproteaceae and Thermococcaceae), or in association with human Apoptosis inhibitor tissues (Pasteurellaceae for oral, Lactobacillaceae for vagina, or Oscillospiraceae for gut). Here, 180 of the 211 families (85% of the total) show a high affinity for at least one environmental type, and 52 (25%) do for just one. This does not imply environmental specificity but does, undoubtedly, indicate a clear environmental preference. The families that are present in many environments, but not showing relevant affinity values for any of them, may be considered ubiquitous.