001, data combined over the 7 months). Soil dilution amendment did not affect plant growth and there learn more were no significant interactions between the factors (dilution, AMF, month of harvest). In the T-RFLP analysis, 68 bacterial TRFs (terminal restriction fragments) were observed

in total: Over the 7 month period 14 TRFs were present in all treatments (i.e. in bare soil, mycorrhizal and non-mycorrhizal planted soils at both dilution treatments across all harvests); 13 TRFs were present only in soils treated with the 10−1 dilution of soil slurry and absent from the 10−6 dilution treatments (planted and unplanted combined) and 14 TRFs were present in the planted treatments and absent from the macrocosms containing bare soil (dilution treatments combined). Six bacterial TRFs were associated with the planted arbuscular mycorrhizal (AM) treatment but not with the planted non-mycorrhizal (NM) treatment. A greater number of fungal TRFs were observed overall (97 TRFs): AZD6244 price over the 7 month period 15 fungal TRFs were present in all treatments; 28 TRFs were observed in planted macrocosms but not in those containing bare soil and 10 fungal TRFs were observed in the planted AM treatments compared

to the planted NM macrocosms. Of the fungal TRFs, 17 were present in soil treated with the 10−1 soil slurry dilution but absent from the 10−6 treatments. In any one dilution/planting regime per month, an overall average (grand mean) of 11 bacterial and 12 fungal TRFs were observed in sufficient

abundance to be included in the analysis. The number of bacterial TRFs identified (TRF richness) was lower in the bare unplanted and the NM planted soils amended with the 10−6 dilution than in the equivalent treatments amended with the 10−1 soil dilution one month after the experiment was established. This trend became less clear over the duration of the investigation until after 7 months the effect of dilution treatment was no longer evident, although TRF richness in the Nintedanib (BIBF 1120) NM soils was greater than in the soil which had AM fungi present (ANOVA: dilution × planting regime × month effect, F6,50 = 3.72, P = 0.004, LSD = 6.3, Fig. 2a). In months 3 and 5, the number of TRFs in the 10−1 AMF treatment was greater than in the 10−6 AMF treatment (data not shown) but by month 7 differences had disappeared ( Fig. 2a). Fungal TRF richness followed similar trends ( Fig. 2b) although data were more variable. Unplanted (bare) soil contained fewer fungal TRFs than planted soils (planting regime, F2,47 = 5.03, P = 0.010) overall. The number of fungal TRFs remained constant over all 7 months whereas the number of bacterial TRFs fell from an average (across all treatments) of 16 in month one to an average of 10 in month 7 (month as a single factor, F3,50 = 15.62, P < 0.001). PCA analysis of the microbial communities illustrated the complexity of these interactive effects.