1974, reviewed in Turrens 2003). However, organisms have also evolved to produce ROS enzymatically in an “oxidative burst.” The oxidative burst is an important component of innate immunity and is conserved among organisms from taxa as distantly related
PLX4032 as all phyla of algae, vascular plants, and animals (Halliwell and Gutteridge 2007). Most ROS act as oxidants (i.e., they are capable of removing electrons from other molecules) and the roles of ROS in various taxa often include toxicity to invading pathogens (Hoffmann et al. 1984, Radi et al. 1991, Peng and Kuc 1992), cross-linking (strengthening) of the cell wall (Bradley et al. 1992, Brisson et al. 1994), and participation in cell signaling which ultimately up-regulates a suite of other defense responses (Levine et al. 1994, Vandenabeele et al. 2003, Soares et al. 2011) and/or promotes healing (Rojkind et al. 2002, Sen and Roy 2008). Reactive nitrogen species (RNS) derived from the reaction of nitric oxide (NO·) with ROS can also be involved in the oxidative burst (Huang et al. 2004, Arasimowicz et al. 2009). The oxidative burst in macroalgae has been well explored in response to pathogen recognition by using pathogen extracts
or pathogen-associated compounds such as lipopolysaccharides as elicitors. It has also been well explored in response to damage recognition by eliciting with compounds produced during the breakdown of the host cell EPZ-6438 datasheet wall such as oligosaccharides derived from agar or alginate (Weinberger Sclareol et al. 1999, Küpper et al. 2001, 2002, Weinberger 2007,
Potin 2008). This type of pathogen recognition-driven oxidative burst in algal, plant, and animal cells, is generally a controlled, receptor-mediated event generated by an enzyme such as an NADPH oxidase or an oligosaccharide oxidase (Babior 1999, Torres et al. 2005, Weinberger et al. 2010). In contrast, the macroalgal production of ROS elicited by direct wounding has rarely been studied, despite the fact that the first observation of a macroalgal oxidative burst was due to wounding (Collén and Pedersén 1994). Reports are limited to the production of H2O2 by the red alga Eucheuma platycladum upon breakage and stirring (Collén and Pedersén 1994) and the production of H2O2 and NO· during wound plug formation in the siphonous green alga, Dasycladus vermicularis (Ross et al. 2006). Wounds provide a potential route of entry for pathogens (Crosse et al. 1972, Jesus et al. 2006), so the oxidative burst elicited by direct wounding may actually be stimulated by pathogen recognition. In other words, wounded macroalgae may respond not to the cellular damage produced by injury but to pathogen-associated compounds, and this response has been studied in detail for some macroalgal species (Potin et al. 2002, Weinberger et al. 2002, Küpper et al. 2006).