It can be noteworthy that cell lines 8505C and B CPAP, which display a close to triploid karyotype with losses at chromosomal region 7q by CGH, present a homozygous pattern for the V600E BRAF mutation. This signifies that the two cytogenetically regular chromosomes 7 observed in each cell lines carry the mutation. Rather than resulting from two independent mutational occasions, this discovering suggests that the BRAF mutation occurred in a single chromosome seven that later on was duplicated and that the 7q using the wild sort BRAF was deleted. However, other candidate genes are tough to high light in this panel of cell lines, as areas recurrently impacted by copy quantity gains and or losses often concerned significant DNA segments. It really should also be noted that continued cell culture tends to produce clonal evolution in vitro, and in particular the tetraploidization phenom ena seems to happen frequently in cell lines over time.
Even when these occasions may generate substantial karyotypic differences in cell line stocks in different laboratories, one would count on that the biologically appropriate oncogenic rearrangements character izing every single cell line are maintained inside the different sub populations. selleckchem Classical cytogenetic evaluation will efficiently detect such clonal divergence, though it calls for consid erable time and experience. An option and less time consuming strategy to be sure cell line identity is genotype analysis employing Quick Tandem Repeat markers. despite the fact that clonal evolution is not going to be detected utilizing this strategy. Certainly, a lately published survey of 40 presumptive thyroid cell lines employing STR markers showed that only 23 cell lines displayed special profiles steady with thyroid origin. with all the remaining cell lines staying derivatives or cross contaminants from distinctive origins, this kind of as melanoma and colon cancer cell lines.
Conclusion We report the genetic background of seven independent thyroid cell lines of papillary, follicular or anaplastic ori gin, selelck kinase inhibitor highlighting distinctive chromosomal markers also as frequent genomic areas of curiosity for future research. Importantly, the literature review signifies these cellular versions show genetic alterations representative of individuals found in clinical tumors from the correspondent histotype. Our findings further remind us that cell line contamina tion transpires far more regularly than a single want to admit, and that karyotyping and or genotyping are effi cient strategies for clonal analysis and determination of cell line identity. We as a result endorse that karyotype and genotype evaluation be performed from early passages and regularly in each and every laboratory functioning with cancer cell lines to assist interpret the genetic information, to detect in vitro clonal evolution with time, and to ensure cell line authenticity.