It truly is noteworthy that cell lines 8505C and B CPAP, which show a near triploid karyotype with losses at chromosomal region 7q by CGH, display a homozygous pattern to the V600E BRAF mutation. This signifies that the two cytogenetically regular chromosomes seven observed in both cell lines carry the mutation. As opposed to resulting from two independent mutational occasions, this discovering suggests the BRAF mutation occurred in one chromosome 7 that later was duplicated and that the 7q with all the wild form BRAF was deleted. Regretably, other candidate genes are hard to large light on this panel of cell lines, as regions recurrently affected by copy number gains and or losses normally involved substantial DNA segments. It need to also be noted that continued cell culture tends to make clonal evolution in vitro, and in particular the tetraploidization phenom ena appears to come about frequently in cell lines more than time.
Even if these occasions might create substantial karyotypic differences in cell line stocks in different laboratories, a single would expect the biologically relevant oncogenic rearrangements character izing each and every cell line are maintained during the distinct sub populations. buy CGK 733 Classical cytogenetic examination will efficiently detect such clonal divergence, though it requires consid erable time and experience. An option and much less time consuming approach to be sure cell line identity is genotype examination utilizing Brief Tandem Repeat markers. though clonal evolution won’t be detected using this technique. Certainly, a just lately published survey of 40 presumptive thyroid cell lines utilizing STR markers showed that only 23 cell lines displayed exceptional profiles steady with thyroid origin. with all the remaining cell lines becoming derivatives or cross contaminants from different origins, such as melanoma and colon cancer cell lines.
Conclusion We report the genetic background of seven independent thyroid cell lines of papillary, follicular or anaplastic ori gin, selleck highlighting distinctive chromosomal markers also as popular genomic regions of interest for long term studies. Importantly, the literature review indicates these cellular models display genetic alterations representative of those located in clinical tumors of the correspondent histotype. Our findings even more remind us that cell line contamina tion transpires much more frequently than 1 would like to admit, and that karyotyping and or genotyping are effi cient methods for clonal evaluation and determination of cell line identity. We for that reason suggest that karyotype and genotype analysis be carried out from early passages and frequently in each and every laboratory functioning with cancer cell lines to aid interpret the genetic data, to detect in vitro clonal evolution with time, and also to make certain cell line authenticity.