Expert subjects were drawn from the small extant community of academic and craft stone toolmakers, and were contacted directly. Imaging sessions for Naive, Trained and Expert FK866 solubility dmso subjects were interspersed over the course of the study. Subjects in all groups received the same instructions before scanning, consisting of a scripted briefing, accompanying PowerPoint presentation, and Cogent script showing instructions and exemplar stimuli (not used in experiment) as presented in

the scanner. Crucially, instructions included a description of the methods and aims of Paleolithic stone toolmaking so that even Naïve subjects had basic conceptual knowledge of the technology. Twenty-second video clips (Supporting Information Video S1) were extracted from full-length videos of an expert toolmaker (right-handed) engaged in Oldowan flaking (n = 6), Acheulean shaping (n = 6) and the Control condition (n = 6). All videos

were recorded on the same day with constant camera position and lighting. The demonstrator was seated facing the camera, and supported the core on his left thigh or above his lap in his left hand. The field of view included this workspace and the full range of arm movements, but did not extend to the face. Talazoparib purchase Flint from a single quarry in Suffolk, UK was used for all toolmaking, and video segments were deliberately selected from early stages of flaking/shaping (e.g. prior to establishment of symmetrical ‘handaxe’ shape) so that size, shape, colour and other large-scale visual characteristics of cores did not differ systematically across stimulus types. Nevertheless, action sequences portrayed in the clips clearly reflected technological differences. Nine types of technological action were identified in the videos, and their frequencies in the actual stimuli used recorded using the EthoLog 2.2.5 behavioural transcription tool (Table 1).

These are: (i) percussive strikes with the right hand; (ii) shifts of the left-hand core grip; (iii) rotations of the core in the left hand; (iv) shifts of the right-hand hammerstone grip; (v) inversions (flipping over) of the core with the left hand; (vi) changing of the hammerstone (here the demonstrator reached off camera to exchange one hammerstone for another, see Supporting Information Video S1); (vii) Carteolol HCl abrasion/micro-flaking of core edges with right hand; (viii) sweeping of detached flakes and fragments off the thigh with the right hand (the hammerstone itself or an extended finger may be used); (ix) grasping of a detached flake or fragment with the right hand to remove it from the thigh, usually with a side-to-side ‘scissor’ grip of index and middle fingers, rarely (twice) with a pad-to-pad ‘pincer’ grip of thumb and index finger (Supporting Information Video S1). Importantly, the total number of actions declines from Control to Oldowan to Acheulean stimuli.