Lmo0096 was also reported as showing lower levels in an L. monocytogenes EGD-e rpoN (σL) mutant in a 2-DE based proteomic analysis [22] and the lmo0096 gene was found to be preceded by a putative σL consensus promoter in the same study, further supporting positive regulation of the gene encoding this protein by σL. Table 2 Proteins found to be differentially regulated by σ L , as determined by a proteomic comparison between L. monocytogenes find more 10403S Δ BCH and Δ BCHL Proteina Fold change Δ BCH /ΔBCHL Description Gene name Role categoryb Sub-Role categoryb Proteins with positive fold change ( > 1.5) and p < 0.05 (indicating positive regulation by σ L ) Lmo0096d,f 64.16 mannose-specific

PTS system IIAB component ManL mptA Energy metabolism Pyruvate dehydrogenase         Amino acid biosynthesis Aromatic amino acid family         Transport and binding proteins Carbohydrates, organic alcohols, and acids Lmo2006g 3.41 acetolactate synthase catabolic alsS Amino acid biosynthesis Aspartate family         Amino acid biosynthesis Pyruvate family Proteins with negative fold change ( < -1.5)

and p < 0.05 (indicating negative regulation by σ L ) Lmo0027c,e −3.62 beta-glucoside-specific PTS system IIABC component lmo0027 Transport and binding proteins Carbohydrates, organic alcohols, and acids         Amino acid biosynthesis Aromatic amino acid family         Energy metabolism Pyruvate dehydrogenase Lmo0130 −3.64 4��8C hypothetical protein lmo0130 Unclassified Role category not yet assigned Lmo0178 −2.07 hypothetical protein lmo0178 Regulatory functions Other Lmo0181 −3.25 multiple Belnacasan in vivo sugar transport system substrate-binding protein lmo0181 Transport and binding proteins Unknown substrate Lmo0260 −1.68 hydrolase lmo0260 Hypothetical proteins Conserved Lmo0278

−1.67 maltose/maltodextrin transport system ATP-binding protein lmo0278 Transport and binding proteins Carbohydrates, organic alcohols, and acids Lmo0319c,e −2.96 beta-glucosidase bglA Energy metabolism Sugars Lmo0343 −3.94 transaldolase tal2 Energy metabolism Pentose phosphate pathway Lmo0344 −4.69 short chain dehydrogenase lmo0344 Energy metabolism Biosynthesis and degradation of polysaccharides Lmo0345 −6.04 ribose 5-phosphate isomerase B lmo0345 Energy metabolism Pentose phosphate pathway Lmo0346 −2.74 triosephosphate isomerase tpiA2 Energy metabolism Glycolysis/gluconeogenesis Lmo0348 −2.41 dihydroxyacetone kinase lmo0348 Fatty acid and phospholipid metabolism Biosynthesis         Energy metabolism Sugars Lmo0391 −1.67 hypothetical protein lmo0391     Lmo0401 −2.16 alpha-mannosidase lmo0401 Unclassified Role category not yet assigned Lmo0517e −3.21 phosphoglycerate mutase lmo0517 Energy metabolism Glycolysis/gluconeogenesis Lmo0521 −2.23 6-phospho-beta-glucosidase lmo0521 Energy metabolism Sugars Lmo0536 −1.97 6-phospho-beta-glucosidase lmo0536 Central intermediary metabolism Other Lmo0574 −1.