None of the

participants took part in Experiment 1. All participants were right-handed as assessed by a German version of the Edinburgh Handedness Inventory (Oldfield, 1971). All had Cell Cycle inhibitor normal or corrected-to-normal vision and did not report any neurological disorder. Participants were reimbursed or received course credits for participation. Two participants were excluded from the analysis due to response accuracy scores below 60% in the sentence-picture-verification task (see Section 3.1.3). Data analysis was thus based on the remaining 19 participants (11 female, M age 25 years, age range 19–30 years). Material for Experiment 2 was identical to Experiment 1. Additionally, 32 colored drawings depicting the scene of the preceding target sentence with correct (matching) or exchanged (mismatching) thematic roles (e.g., The owl paints the hedgehog. vs. The hedgehog paints the owl.) were created for the sentence-picture-verification

task. For each of the four selleck kinase inhibitor experimental conditions (NEUTRAL SO/OS, TOPIC SO/OS) the same number of matching/mismatching pictures was constructed. The procedure was identical to that of Experiment 1 except for the following three methodological adjustments: First, the participant was prepared for EEG recording prior to the experiment. Second, presentation of the target sentence was preceded and followed by a fixation cross for 500 ms in the center of the screen to reduce vertical eye movements of the participant. Third, instead of the behavioral judgment task on story comprehensibility, the participants performed a sentence-picture-verification task that followed the target sentence in 20% of the trials: After offset of the fixation cross, which followed the target sentence, the matching/mismatching picture was presented for 2 s before

the participant had to press the corresponding button (yes vs. no) within a time window Cyclooxygenase (COX) of 2 s. The assignment of the response buttons to the right index and middle fingers was counterbalanced across participants. A written instruction informed participants to read each scene attentively and silently and to answer the sentence-picture-verification task as accurately and fast as possible. Participants were asked to sit in a relaxed manner and to avoid blinks as well as other movements during sentence reading. The whole experimental session including three practice trials and pauses after each of the 40 trials lasted approximately 30 min plus electrode preparation. The EEG was recorded through a 32 channel active electrode system (Brain Products, Gilching, Germany) fixed at the scalp by means of a soft cap (Easycap, Inning, Germany). The electrode configuration included the following 29 scalp sites according to the international 10–20 system (American Electroencephalographic Society.