PBO-4

is a putative Na+/H+ ion exchanger expressed on the basolateral membrane of the intestine, juxtaposed to the posterior body muscles. In pbo-4 mutants the extracellular space is not acidified and the muscles fail to contract. The pbo-5 and pbo-6 genes encode subunits of a “cysloop” proton-gated cation channel required for muscles to respond H 89 to acidification. In heterologous expression assays the PBO receptor is half-maximally activated at a pH of 6.8. The identification of the mechanisms for release and reception of proton signals establishes a highly unusual mechanism for intercellular communication.”
“3-Hydroxy-3-methylglutaryl coenzyme A reductase inhibitors (statins) exert pleiotropic effects on the cardiovascular system, in part through a decrease in reactive oxygen species (ROS) formation and reduction of vascular inflammation. To elucidate the molecular mechanisms involved in these effects, we investigated the effect of statins on TNF-alpha-induced ROS production, vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) expression

in human aortic endothelial cells (HAECs). Exposure www.selleckchem.com/products/AZD8055.html of HAECs to TNF-alpha caused production of ROS via Rac-1 membrane translocation and activation. The Rac-1 activation and ROS liberation mediated TNF-stimulated NF-kappa B activation and the subsequent VCAM-1 and ICAM-1 expression. Extracellular-signal-regulated kinase 5 (ERK5) plays a central role in inhibiting endothelial inflammation. Immune complex kinase assay of protein extracts from HAECs treated with atorvastatin revealed increased ERK5 activity in a time- and dose-dependent manner. In addition, pretreatment with atorvastatin inhibited TNF-alpha-induced BIIB057 solubility dmso ROS production and VCAM-1 and ICAM-1 expression. Chemical or genetic inhibition of ERK5 ablated the statins inhibition of Rac-1 activation,

ROS formation, NF-kappa B, VCAM-1 and ICAM-1 expression induced by TNF-alpha. Taken together, statins, via ERK5 activation, suppress TNF-stimulated Rac-1 activation, ROS generation, NF-kappa B activation and VCAM-1 and ICAM-1 expression in human ECs, which provides a novel explanation for the pleiotropic effects of statins that benefit the cardiovascular system. 2013 Elsevier Inc. All rights reserved.”
“Objectives: To examine the sexual behaviors and reproductive concerns among patients with moderate to complex congenital heart disease (CHD).\n\nBackground: There is a growing need to understand and address the psychosocial issues for older adolescents and young adults with CHD. Emerging sexuality is an issue for this age group and pregnancy for many women with CHD is risky. But, patients’ sexual behavior and reproductive concerns have not been studied.\n\nMethods: Young adults (19 – 20 years old; n=212) and adolescents (16 – 18 years old; n=144) with moderate to complex CHD reported their sexual behaviors and reproductive concerns. Data were compared to non-native samples from Canada and the United States.