PBP2a detection was performed using monoclonal PBP2a antibody (1:

PBP2a detection was performed using monoclonal PBP2a antibody (1:20000) from the MRSA-screen kit (Denka Seiken). Acknowledgements We would like to thank Frances O’Brien (School of Biomedical Sciences, Curtin University of Technology) for determining the MLST types of strains ZH44 and ZH73. We would also like to thank Sibylle Burger for technical assistance and Dr. P. Hunziker, of the Functional Genomics Centre

Zurich, University of Zurich, for protein analysis. We are also grateful to T. Bae (Department of Microbiology, University of Chicago) for providing the plasmid pKOR1. This study was supported by the Swiss National Science Foundation grant NF31-117707/1. References 1. Kirby WMM: Extraction of a highly potent penicillin inactivator from penicillin resistant styaphylococci. Science 1944,99(2579):452–453.CrossRefPubMed 2. Hartman PLX-4720 order BJ, Tomasz A: Low-affinity penicillin-binding protein GDC-0973 supplier associated with beta-lactam resistance in Staphylococcus aureus. J Bacteriol 1984,158(2):513–516.PubMed 3. Reynolds PE, Brown FJ: Penicillin-binding proteins of β-lactam-resistant strains of Staphylococcus aureus . Effect of growth conditions. FEBS Lett 1985,192(1):28–32.CrossRefPubMed 4. Lim D, Strynadka NC: Structural basis for the β-lactam resistance of PBP2a from methicillin-resistant Staphylococcus aureus. Nat Struct Biol 2002,9(11):870–876.PubMed 5. Sharma VK, Hackbarth CJ, Dickinson

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