Platelet depletion in plasma samples produced no differences of anti-VEGF titers in serum and plasma for each animal, for all the evaluated conditions. The ability of serum to block the interaction of KDR-Fc with human VEGF was assessed using an ELISA assay. As shown in Fig. 3, all immunized animals evidenced a significant increase of the inhibition of VEGF/KDR-Fc binding as compared to the placebo group, at a 1:50 sera dilution (p < 0.05, One way ANOVA, Bonferroni post-test). A significant lower inhibition was associated with animals included in the biweekly schedules as compared to those IWR 1 immunized

every week (p < 0.05, One way ANOVA, Bonferroni post-test). Wound closure dynamics were studied using a standard cutaneous round deep ulcer model. As can be seen from Fig. 4A and B, no differences were detected in the healing indexes of wounds of immunized animals as compared with placebo-treated animals. Histological verification of wound tissue showed full healing in all animals. All animals appeared generally healthy during the vaccination period. No changes 3-deazaneplanocin A clinical trial in overall behavior, feeding, neuromuscular performance, body weight or appearance of fur in immunized animals, were reported. Animals were sacrificed and organs weight and appearance

recorded. No differences in uterus or ovary weight were reported for CIGB-247 immunized rats as compared to control groups. No changes were detected after careful histological examination of heart, trachea, spleen, adrenal glands,

liver, kidney and ovaries (follicle maturation or presence/absence of corpus luteum), and for possible thrombosis effects or bleeding (results nor shown in detail). Fig. 5 Ketanserin shows that anti-human VEGF IgG antibody titer kinetics resembled the scenario described above for rats. The weekly scheme proved slightly better than the biweekly vaccination in terms of antibody titer. Addition of montanide to the latter led to the highest titers of the experiment. One booster in the weekly scheme was sufficient to regain titer values obtained after the induction phase. The ability of serum to block the interaction of KDR-Fc with human VEGF was estimated using the designed ELISA assay, this time with a 1:500 serum dilution. All immunized groups exhibited high and similar inhibition values, as compared to placebo-treated animals (Fig. 6). All animals appeared healthy during immunization, without changes in behavior, feeding, body weight or appearance of fur. No changes in hematologic or blood biochemical parameters were observed. Animals were sacrificed and organs weight and appearance recorded. No changes were detected; particularly no differences in uterus or ovary weight were reported for CIGB-247 immunized rabbits as compared to control animals.