Strain KT71T was subsequently characterized in detail and described as Congregibacter litoralis by Spring et al, therefore representing the primary photoheterotrophic bacter ium of this group which has a validly published title. Phylo genetically, C. litoralis is affiliated to a big coherent cluster of 16S rRNA gene sequences, which had been mainly retrieved by cultivation independent strategies from marine habitats around the globe.
This sequence clus ter was acknowledged being a distinct lineage inside of the class Gammaproteobacteria and designated as OM60 or NOR5 clade, Metabolic lively bacteria representing this clade could be detected in quite a few environmental selleck samples by using fluorescence in situ hybridization experiments, Based mostly on these come across ings it can be assumed that the OM60 NOR5 clade of Gammaproteobacteria is of major ecological im portance as a consequence of its widespread occurrence during the eupho tic zone of saline ecosystems and high abundance in particular in coastal waters, A phylogenetic lineage closely linked on the OM60 NOR5 cluster was originally defined by a 16S rRNA gene sequence retrieved from deep sea sediment and designated BD1 seven, In recent years reports with regards to the isolation of add itional strains belonging on the OM60 NOR5 group have accumulated. A few of these strains had been described as mixotrophs containing photosynthetic pigments or proteorhodopsin, In contrast, no photosyn thetic pigments were reported in members with the genus Haliea or Halioglobus, The not long ago de scribed non pigmented species Dasania marina and Oceanicoccus sagamiensis are almost certainly affil iated to the BD1 7 lineage, whereas representatives on the even more distantly associated genera Spongiibacter and Zhongshania form a third phylogenetic branch.
In this research, a thorough phenotypic and genotypic characterization within the novel isolate Ivo14T was performed that allowed a comprehensive comparison to other bacteriochlorophyll a containing members of your OM60 NOR5 clade, in order that a profound understanding from the metabolic plasticity and taxonomic relationships en countered on this ecologically essential group of marine could possibly be AV-412 obtained. Benefits and discussion Isolation and identification of mixotrophic representatives in the OM60 NOR5 clade An isolation system originally designed for the retrieval of strains belonging to the genus Rhodopirellula inside the Planctomycetales resulted from the isolation of numer ous representatives within the OM60 NOR5 clade of marine gammaproteobacteria, The isolation method in cluded using antibiotics and also a screening of red pigmented strains, so that all retrieved OM60 NOR5 isolates were pigmented.
Strains belonging to this phylo genetic group represented about 10% of all red pigmented colonies and could be affiliated both for the NOR5 three or NOR5 one lineage within this clade based mostly on analyses of their 16S rRNA gene sequences, Strains belonging to the OM60 NOR5 clade had been even further exam ined for your presence of pufL and pufM genes encoding proteins from the photosynthetic response center.