Streptomyces suspension VX-770 concentration cultures were grown three days in ISP-2 medium. From the tester strain, 40 μl of this suspension culture was applied on the lower part of an agar filled Petri dish, forming a line. After the sporulation of the tester strain begun, 3 parallel lines of the receiver strain were applied perpendicularly to the tester line. For
each Streptomyces pair, three tester and nine receiver lines were applied. The impact of the tester strain on the formation of receiver strain’s substrate Selleckchem Eltanexor mycelium and sporulation was recorded at the time point of the onset of sporulation in the control cultures. Impact of Streptomyces culture filtrates and culture extracts on non-streptomycetous bacteria Pure culture filtrates and organic extracts of streptomycetes were tested against bacteria. Streptomyces suspension cultures were grown three days in ISP-2 medium. To obtain pure culture filtrate, the cells were centrifuged (3800 rpm, 10 min), and the supernatants were filtered (0.45 μm). Organic extracts were prepared Fedratinib chemical structure from the pure culture filtrates, which were adjusted to pH 5.0 and extracted 1:1 (vol/vol) with ethyl acetate. The organic phase was concentrated to dryness using a vacuum evaporator and re-dissolved in 1/10 of the
original volume in ethanol. Gram-positive bacteria (Bacillus subtilis DSM 10, Staphylococcus aureus DSM 20231, Mycobacterium phlei DSM 750) and Gram-negative bacteria (Escherichia coli K12 (W1130), Pseudomonas fluorescens DSM 50090) were tested. Bacillus subtilis DSM 10 was initially cultured in DSMZ 1 medium at 37°C and tested on DSMZ 1 and MM 1 agar media. Staphylococcus aureus DSM 20231 was initially cultured in KM 1 medium at 37°C and tested on KM 1 agar medium. Mycobacterium phlei DSM 750 was initially cultured in KM 1 medium at 27°C and tested on KM 1 agar medium. Escherichia coli K12 (W1130) was initially Astemizole cultured in KM 1 medium at 37°C and tested on KM 1 and MM 1 agar media. Pseudomonas fluorescens
DSM 50090 was initially cultured in KM 1 medium at 27°C and tested on KM 1 and MM 1 agar media. KM 1 medium consisted of 8 g Difco nutrient broth, 5 g NaCl, 20 g agar per 1 liter of de-ionized water. The pH was adjusted to pH 7.2 prior to sterilization. KM 5 medium consisted of 4 g yeast extract, 10 g malt extract, 4 g glucose, 20 g agar per liter un-distilled water. The pH was adjusted to pH 5.5 prior to sterilization. DSMZ1-medium consisted of 5 g Bacto peptone, 3 g malt extract, 10 mg MnSO4 x H2O and 20 g agar per liter of un-distilled water. The pH was adjusted to 5.5 prior to sterilization. MM1 medium  consisted of 5 g glucose, 0,5 g tri-sodium-citrate x 2 H2O, 3 g KH2PO4, 7 g K2HPO4, 0.1 g MgSO4 x 7 H2O, 1 g (NH4)2SO4 and 15 g Bacto agar.