Stroma white inside Spore deposits white to pale yellowish Rehy

Stroma white inside. Spore deposits white to pale yellowish. Rehydrated buy SCH772984 stromata smooth, yellowish to pale ochre, ostiolar dots convex, intensely ochre to light brown, 100–160(–210) μm diam. After addition of 3% KOH macroscopically light brown, without a colour change, under the stereo-microscope more orange

and fine pigment stripes more distinct, often concentric around the ostioles. Stroma anatomy: Ostioles (50–)57–75(–90) μm long, projecting to 25 μm, (40–)47–68(–76) μm wide at the apex (n = 30), short-cylindrical, periphysate, sometimes lined at the apex by subglobose or apically pointed, hyaline cells 5–9(–14) μm wide. Perithecia (190–)260–320(–340) × (120–)160–240(–285) μm

(n = 30), crowded, flask-shaped, ellipsoidal or globose; Selleckchem ABT-263 peridium (15–)18–25(–28) μm (n = 30) thick at the base, (10–)13–19(–22) μm (n = 30) at the sides, yellow. Cortical layer (18–)24–38(–44) JPH203 price μm (n = 30) thick, a t. angularis of distinct, thin- or thick-walled cells (3.5–)6–14(–23) × (3–)5–9(–10) μm (n = 60) in face view and in vertical section, subhyaline, yellow to orange, with inhomogeneously distributed pigment, around the ostioles typically smaller and in parallel rows. Subcortical tissue variable, mostly a t. intricata of hyaline, thin-walled hyphae (2–)4–6(–7) μm (n = 30) wide, or a t. angularis of hyaline, thin-walled cells (3–)5–9(–15) × (3–)4–7(–8) μm (n = 30). Subperithecial tissue an ill-defined t. intricata of hyaline, thin-walled hyphae (2.5–)4–9(–12) μm (n = 40) wide. Asci (63–)80–98(–112) × (4.5–)4.7–5.5(–6.0) μm, stipe 5–18(–34) μm long (n = 90), apex with a minute flat ring, Cytidine deaminase base with crozier. Ascospores hyaline, verruculose or spinulose with spines to 0.5 μm long; cells dimorphic; distal cell (3.0–)3.5–4.0(–5.5) × 3.0–3.5(–4.2) μm, l/w (0.9–)1.0–1.3(–1.7) (n = 120), (sub)globose, sometimes wedge-shaped at

the apex; proximal cell (3.2–)4.0–4.8(–5.5) × (2.2–)2.7–3.0(–4.0) μm, l/w (1.2–)1.4–1.7(–2.1) (n = 120), oblong, ellipsoidal or plump wedge-shaped, sometimes subglobose. Cultures and anamorph: growth rate only studied in a single experiment using a single isolate; optimal growth at 25°C on all media; at 30°C hyphae dying after a short initial growth of max. 0.5 mm; no growth at 35°C. On CMD after 72 h 8 mm at 15°C, 11 mm at 25°C; mycelium covering the plate after 17 days at 25°C. Colony hyaline, thin, circular, indistinctly broadly zonate, margin diffuse; hyphae with little variation in width. Aerial hyphae inconspicuous, loose, several mm long and high. Autolytic activity absent, coilings rare. No chlamydospores seen. No diffusing pigment, no distinct odour noted. Conidiation noted after 10 days as scant conidia on aerial hyphae.

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