21. Trichoderma solani Samuels, V. Doyle et V. S. Lopez, sp. nov. Figs. 3h, i and 17. Fig. Autophagy Compound Library price 17 Trichoderma solani. a, b Young pustules, conidia just beginning to turn green. c–h Conidiophores. i Conidia. All from G.J.S. 88–81. Scale bars: a = 1 mm, b =250 μm, c–f = 20 μm, g–i = 10 μm MycoBank MB 563912 Conidiophora verticillate ramosa. Phialides lageniformes, ad apicem in collula brevia constrictae. Conidia ellipsoidea, 2.5–2.7(−3.0) × 1.7–2.2 μm,
laevia, atroviridia. Incrementum tardum; in agaro dicto PDA ad temperaturam 20–30°C post 96 h radius coloniae ca. 25 mm, colonia lutescens. Holotypus: BPI 882298 Teleomorph: none known Optimum temperature for growth on PDA 20–30°C, on SNA 25–30°C; after 96 h in darkness with Epigenetics intermittent light colony radius on PDA at 20–30°C ca. 25 mm, on SNA at 25–30°C 15–20 mm; at 35°C
after 96 h colony radius less than 10 mm on PDA, less than 5 mm on SNA. selleck compound Conidia forming on PDA within 72 h at 30°C, within 96 h at 20–25°C; diffusing yellow pigment forming on PDA within 48 h at 25–30°C. Colony on PDA after 1 week at 25°C under light with a scalloped margin; conidia forming over the whole surface of the colony in zonate rings, gray-green, surface disposed in rays; at 35°C conidia covering nearly the entire colony. Colonies grown on SNA in darkness with intermittent light sterile after 96 h; conidia forming within 1 week at 25°C under light in 1–2 mm diam, flat pustules in the center of the colony; individual conidiophores visible in pustules; pustules formed of intertwined hyphae, typically comprising a distinct central axis with frequently paired fertile lateral branches, the lateral branches distal to the tip longer than branches proximal to the tip; phialides arising directly
from lateral branches, the longer lateral branches re-branching in pairs, the short secondary branches typically consisting of a single cell and terminating in a whorl of 2 or 3 phialides; intercalary phialides not seen. Phialides (n = 30) lageniform, (4.7–)5.5–8.5(−10.2) μm long, (1.7–)2.2–3.0(−4.2) μm at the widest point, L/W 1.9–3.5(−4.6), base (1.0–)1.2–2.0(−2.5) μm wide, arising from a cell (1.5–)2.0–2.5(−3.2) Branched chain aminotransferase μm wide. Intercalary phialides not seen. Conidia (n = 30) ellipsoidal, (2.0–)2.5–2.7(−3.0) × 1.7–2.2(−2.5) μm, L/W (1.1–)1.2–1.4 (95% ci: 2.5–2.6 × 2.0–2.1 μm, L/W 1.2–1.3), dark green, smooth. Chlamydospores not observed. Etymology: ‘solani’ refers to the host from which this species was isolated, Solanum hintonii. Habitat: endophytic in tubers of Solanum hintonii. Known distribution: Mexico, known only from the type locality. Holotype: México, Estado de México, 6.5 km from junction of road from Temascaltepec towards San Pedro Tenayac, W of stream and 150 m N of the road, 19.05041 N, 100.10523 W, 25 Jul 2007, isolated as an endophyte from tubers of Solanum hintonii, V.