No difference was found between the areas of cartilage measured by the two methods (p=0.999). Callus area measured by CECT was smaller than, but strongly predictive of (R2=0.80, p<0.001), the corresponding histomorphometric measurements. CECT also enabled qualitative identification of mineralized cartilage. These findings indicate that the CECT method provides accurate, quantitative, and non-destructive visualization of the shape and composition of the fracture callus, even during the early stages of repair when little mineralized tissue is present. The non-destructive nature of this method
would allow subsequent analyses, such as mechanical testing, to be performed on the callus, thus enabling higher-throughput, comprehensive investigations of bone healing. (c) see more 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 31: 567573, 2013″
“Objective: To examine the potential contribution of the serotonin hydroxylase (TPH1 and TPH2) genes, and the serotonin transporter promoter polymorphism (5HTTLPR) Fer-1 mw to the unique and pleiotropic risk of PTSD symptoms and depressive symptoms.\n\nMethods: Participants included 200 adults exposed to the 1988 Spitak earthquake
from 12 multigenerational families (3 to 5 generations). Severity of trauma exposure, PTSD, and depressive symptoms were assessed using standard psychometric instruments. Pedigree-based variance component analysis was used to assess the association between select genes and the phenotypes.\n\nResults: After adjusting for age, sex, exposure and environmental variables, there was a significant association of PTSD symptoms with the ‘t’ allele of TPH1 SNP rs2108977 (p<0.004), explaining 3% of the phenotypic variance. This allele also showed a non-significant trend for
an association with depressive symptoms (p = 0.08). Epigenetic inhibitors Also, there was a significant association of PTSD symptoms and the ‘t’ allele of TPH2 SNP rs11178997 (p = 0.03), explaining 4% of the variance. Depressive symptoms were significantly associated with the ‘s’ allele of 5H11′LPR (p = 0.03), explaining 4% of the variance. Limitations: Retrospective rating of exposure may have been subject to memory failure leading to misestimation of symptom seventies. Second, findings may not be generalizable to other ethnic/racial populations.\n\nConclusion: To our knowledge, this is the first published report showing that variants in TPH1 and TPH2 genes constitute risk factors for PTSD symptoms. Additionally, the TPH1 gene may be associated pleiotropically with PTSD and depressive symptoms. The association of the ‘s’ allele of 5HITLPR polymorphism with depression adds to similar findings from case/case-control studies. (C) 2012 Elsevier B.V. All rights reserved.