Two EOC cell lines, MDAH-2774 and SKOV-3, were cultured under normoxic (20% O(2)) and hypoxic (2% O(2)) conditions using standard techniques. After EOC cells
were transfected with siRNA, HIF-1 alpha and VEGF mRNA levels were measured by real-time RT-PCR. Angiogenesis was evaluated utilizing LOXO-101 concentration an in vitro assay model consisting of human umbilical vein endothelial cells (HUVEC) and polymerized ECM Matrix.
Both EOC cell lines evaluated constitutively expressed HIF-1 alpha and VEGF mRNA. HIF-1 alpha and VEGF mRNA levels were significantly increased in response to hypoxia (P < 0.05). Under hypoxic conditions, inhibition of HIF-1 alpha gene expression by a specific siRNA resulted in a significant reduction in HIF-1 alpha and VEGF mRNA levels (P < 0.05). In the in vitro angiogenesis model, supernatant from the hypoxic EOC cells induced the HUVEC to form a complex tubular network, a hallmark of angiogenesis. Semi-quantitative analysis of the angiogenesis
assay revealed Selleck FG4592 a significant reduction in tube formation when supernatant from HIF-1 alpha siRNA-treated hypoxic EOC cell was used (P < 0.05).
Inhibition of HIF-1 alpha expression by specific siRNA resulted in a significant decrease in VEGF production and angiogenesis. Further investigation of HIF-1 alpha inhibition for anti-tumor activity is warranted and may potentially prove HIF-1 alpha as a therapeutic target in the management
ovarian cancer.”
“A novel highly efficient beta-nucleating agent for isotactic polypropylene (iPP), hexahydrophthalic barium (HHPA-Ba), was found and its effects on the mechanical properties, the beta-phase content, and crystallization behavior of iPP were investigated, respectively. The results show that the beta-phase content of nucleated iPP (k(beta) value) can reach 80.2% with 0.4 wt % HHPA-Ba. The impact strength and crystallization peak temperature of nucleated iPP are greatly increased. Compared with pure iPP, the impact strength of nucleated iPP can increase 2.4 times. Meanwhile, the spherulite size of nucleated iPP is dramatically decreased than that Selleck Nec-1s of pure iPP. The Caze method was used to investigate the nonisothermal crystallization kinetics of nucleated iPP and the crystallization active energy was achieved by Kissinger method. (C) 2011 Wiley Periodicals, Inc. J Appl Polym Sci 123: 108-117, 2012″
“We have fabricated a rechargeable photo-chemical battery using ion-hole conducting AgAsS2 films. An asymmetrically-electroded Al/AgAsS2/SnO2 yields photocurrents and upon illumination it is concomitantly charged up. When the illumination is stopped, the stack discharges electricity into an external circuit. This photo-charging and discharging phenomenon is attributed to a photo-chemical modification of Ag-ion distribution in amorphous AgAsS2. (C) 2011 American Institute of Physics. [doi:10.1063/1.