These proteases may cleave extracellular matrix proteins and injure the endothelium. Lu et al. demonstrated that

ANCA-activated neutrophils released serine proteases, but not superoxide when co-cultured with EC, and that serine proteases mediated EC damage resulting in von Willebrand factor (vWF) release [78]. Serine proteases that are packaged in ANCA-induced neutrophil microparticles or in neutrophil extracellular check details traps (NETs) possibly also participate in endothelial damage [79,80]. Together, ANCA induce a variety of neutrophil responses in vitro. Some of these were shown to be significant in vivo, such as p38 MAPK, PI3Kγ, C5a and serine proteases. Others that are thought to be important await further in-vivo proof, including the role of ANCA-induced reactive oxygen generation. The neutrophil is both the cell that expresses target

ANCA antigens and a major effector cell in ANCA-induced small vessel vasculitis. The ANCA antigens PR3 and MPO differ substantially in their expression pattern on the neutrophil plasma membrane. ANCA bind to membrane expressed target antigens and initiate intracellular signalling events. The PR3–NB1–Mac-1 membrane complex is one example showing that larger signalling complexes with transmembrane molecules exist. Distinct signalling pathways triggered by ANCA F(ab)2 and the intact ANCA IgG molecule were identified and co-operate in neutrophil activation. Detailed characterization LY2606368 supplier of the activation process will identify novel treatment targets that need to be tested in animal models and subsequently in patients. Ralph Kettritz was supported by grants from the Deutsche Forschungsgemeinschaft and the Experimental and Clinical Research Center, a joint co-operation between the Charité Medical Faculty and the Max-Delbrück Center for Molecular Elongation factor 2 kinase Medicine Berlin-Buch. Nothing to declare. “
“Cytomegalovirus (CMV) -specific immunity is often estimated by the number of in vitro CMV antigen-inducible interferon-γ-positive

(IFN-γ+) T cells. However, recent work indicates that simultaneous production of IFN-γ, tumour necrosis factor-α (TNF-α) and interleukin-2 (IL-2) (referred to as ‘polyfunctionality’) is more relevant for anti-viral protection. Here, we compared polyfunctionality of CMV-specific T cells (pp65 and IE-1 proteins) in 23 solid-organ transplant patients and seven healthy controls by flow cytometry. The proportions of TNF-α+/IFN-γ+/IL-2 cells among the activated cells were significantly reduced in transplant patients but not the frequencies of IFN-γ+ CD8+ T cells. Immunosuppression reduces polyfunctionality, which reflects the increased infection risk in this patient group. In healthy individuals, CD4+ and CD8+ T cells restrain many infectious pathogens but in transplant patients these mechanisms are weakened by the immunosuppressive medication required to prevent graft rejection.