, 2007). Kerksick et al. (2007) suggested that intensive resistance-training reduces the availability of essential amino acids, which kinase inhibitor Bicalutamide in turn, may decrease the rate of tissue repair and growth. Ingestion of whey protein via post training supplementation would subsequently generate a rapid increase in the plasma volume levels of amino acids, producing elevated protein synthesis, and little change in protein catabolism (Kerksick et al., 2006). Whey protein supplementation is purported to elicit a higher blood amino acid peak and prevent protein degradation (Kerksick et al., 2007). The amount of whey protein in our study (i.e. 60 g/d) was higher compared to other studies on multi-ingredient supplementation and resistance training (13 g serving (Chromiak et al., 2004); 7 g serving (Schmitz et al.

, 2010) or comparable (Burke et al., 2001)). In that study, Burke et al. (2001) found no effect on knee flexion peak torque, 1-RM for the bench press and squat exercises were unaffected. The amount of HMB in our study (3 g/d) was similar to the study by Panton et al. (2000). HMB is a metabolite of the essential amino acid leucine. It may enhance gains in strength associated with resistance training (Slater and Jenkins, 2000). HMB has been suggested to act as an anti-catabolic agent, minimizing protein degradation, and muscular cell damage as a result of high-intensity resistance-training, stimulating increased gains in strength. It was reported that short-term HMB supplementation during resistance training significantly enhanced upper body strength (Panton et al., 2000).

Not all research supports gains in muscular function with HMB supplementation (for a review see Wilson et al., 2008). During 4-weeks of HMB supplementation, in comparison to a placebo, no significant changes in strength, expressed as gains in total weight lifted in a maximal repetition test at a load equal to 70% of 1RM, for the BP, squat, and power clean exercises were reported (Kreider et al., 1997). It was concluded that HMB supplementation during training provides no ergogenic value to experienced resistance-trained athletes (Kreider et al., 1997). Although our groups had at least one year of experience with resistance training exercises, our group of participants could not be considered experienced resistance-trained athletes.

Besides creatine monohydrate, whey protein and HMB, Cyclone contains ingredients for which there is no strong evidence to be beneficial for enhancement of strength and/or endurance adaptations by resistance training. Glutamine has been suggested GSK-3 to enhance protein synthesis and minimise catabolic responses during heavy resistance-training, increasing muscular hypertrophy, and reducing exercise-induced immunosuppression (Kreider, 1999) but others reported no effect of glutamine supplementation in combination with a six-week resistance-training program (Candow et al., 2001).

According to Barbosa et al. (2009), the use of aquatic cycling has been reported in literature for three decades, though its findings are still contradictory. Alberton et al. (2010) suggest that HR in the water could be similar or higher as compared with dry land measurements. Barbosa et al. (2010) analyzed the relationships selleck chemical between musical cadence and the physiological adaptations to basic head-out aquatic exercises. The study included an intermittent and progressive protocol and the main conclusion was that increasing musical cadence imposed an increase in the physiological response. In this context, several physiologic indicators have been used in order to quantify the intensity of exertion in those environments, such as: the HR (Sheldahl et al., 1984; Reilly et al., 2003); double product (Veloso et al.

, 2003), and blood lactate concentration (Di Masi et al., 2007). In water, resting or exercising induces different physiological responses when compared with those achieved in dry-land conditions (Shono et al., 2000; Reilly et al., 2003) and are affected by a number of factors, such as buoyancy, thermal conductivity of the water (Choukroun and Varene, 2000), hydrostatic pressure (Goodall and Howatson, 2008), among others. Those responses depend also on the body positioning in the water (Millet et al., 2002; Ega?a et al., 2006) and on the type of exercise (Barbosa et al., 2009). Kang et al. (2005) compared the responses of HR between intermittent (130 �� 2 bpm) and continuous cycling (127 �� 2 bpm) on land and did not found significant differences between both methods.

The lactate concentration was significantly higher at the end of the intermittent exercise with a mean value above 7 mmol in the final stage of the IP. Contrarily, Sabapathy et al. (2004), have examined the physiological responses in 10 subjects who performed a continuous and intermittent land cycling protocol and observed that the intermittent protocol was associated to significantly lower values of HR. Unfortunately, no previous study examined the type of physiological response induce by continue or intermittent exercise in water environment. Therefore, the present study tested the hypothesis that the type of exercise (continuous vs. intermittent) would affect the physiological response and the perception of effort during aquatic cycling. Methods Participants Ten women (values are mean �� SD: age=32.

8 �� 4.8 years; height=1.62 �� 0.05 cm; body mass=61.60 �� 5.19 kg; estimated body fat=27.13 �� 4.92%) of low risk, practicing regular classes of cycling in water for at least six months, participated in the study. All of them signed a written informed consent to participate in Brefeldin_A the study and in accordance with the norms for accomplishment of research with humans established in the Helsinki Declaration of 1975. The experimental procedures were approved by the Ethics Committee of the Institution.

2c). Four seconds after the initial MVC, PT was 62.6 �� 10.8 Nm, a 45 �� 13% increase compared to the pre-MVC value (Figure 2a). There was a sharp decline in PT in the following 60 s so that PT after 2 min was not selleck chemicals Pazopanib significantly different (p>0.05) from the pre-MVC PT (Figure 2a). However, PT returned to baseline pre-MVC value only after 6 min. Figure 2 Time decay of PT (a), RTD & CT (b), and RR & ?RT (c) after a 5 s MVC in response to electrical stimulation reported as % change from unpotentiated values for study 1. * p< 0.05 for unpotentiated values. PT, peak twitch ... RTD and RR increased significantly (p<0.05) by 53 �� 13% and 50 �� 17%, respectively, immediately after the MVC whilst CT and ?RT were unchanged for the duration of the experiment (Figures 2b and and2c).2c).

RTD and RR returned to the pre-MVC values within 3 min after the initial MVC. The decay in PT was associated with a progressive fall in the RTD and in the RR (Figures 2b and and2c).2c). Correlation between PT vs RTD, PT vs RR and PT vs CT was r2 = 0.99 (p<0.001), 0.98 (p<0.001) and 0.56 (p<0.01), respectively, during the 10 min period after the MVC. EMD did not change at any time during this section of the experiment (data not shown). Study 2 Unpotentiated muscle: Torque response to repeated SS over 1 min SS torque response to the first 6 episodes of electrical stimulation (Figure 1c) delivered to the unpotentiated muscle in the min prior to the first MVC did not differ from each other (p>0.05) and the mean values did not differ from those of study 1. Mean values for PT, EMD, CT, ?RT, RTD and RR were respectively 43.

5 �� 12.9 Nm, 34.2 �� 3.1 ms, 85.9 �� 9.5 ms, 80.3 �� 10.5 ms, 0.52 �� 0.18 Nm/ms and 0.56 �� 0.21 Nm/ms (Table 2). Table 2 Responses of single stimulus at specific time points at rest for study 2 (n= 6) Potentiated muscle: Torque response to repeated SS after 10 MVCs PT immediately (4 s) after the first MVC (MVC 1) was increased by 56 �� 10% (Figure 3a) to 67.0 �� 17.7 Nm. PT immediately after MVCs 2�C10 was not different (p>0.05) from PT immediately after MVC 1 (Figure 3a). Figure 3 Time decay of PT (a), RTD & CT (b) and RR & ?RT (c) after a 5 s MVC in response to electrical stimulation reported as % change from unpotentiated values for study 2. * p< 0.05 from MVC 1. Other values were not different ... PT then decayed from 4�C45 s after each MVC so that at 16 s after MVC 1, PT fell significantly (p<0.

001) from the 4 s value PT, but PT was still 29 �� 7% above the unpotentiated value after 45 s. Interestingly the following MVCs showed similar PT at 4 s after MVC, but PT was significantly (p<0.05) higher 30 and 45 s after MVC 2 and 8, 12, 16, 30 and 45 s after MVC 5 and 10 compared to MVC 1, indicating a slower decay GSK-3 of PT (Figure 3a). In addition PT at 45 s after the first MVC was significantly lower (p<0.05) than were the values 45 s after any of the following MVCs (2�C10).

001) and plasma ET-1 at the end of exercise (p<0.01) in all subjects. The values of ADM, NA, and A obtained at the 6th minute of exercise were significantly higher than those at the 3rd minute (p<0.001). At the 5th min of the recovery period, plasma ADM was significantly higher than that before exercise whereas selleckchem plasma NA, A and ET-1 concentrations did not differ significantly from the resting values (Fig. 2). Figure 2 The plasma concentrations of adrenomedullin, noradrenaline, adrenaline and endothelin-1 at rest, during handgrip (3�� and 6��) and at the 5thmin of the recovery period (rec). Values are means �� SEM; * p<0.05, ** p<0.01 ... Significant positive relationships were ascertained between baseline values of plasma ADM and NA concentrations (r= 0.650, p<0.

001), and between the exercise-induced increases in plasma ADM (expressed as percentage of baseline values) and those in NA and ET-1 concentrations (r= 0.710, p<0.001; r= 0.680, p<0.001; respectively). The exercise-evoked increases in plasma ET-1 concentrations (expressed as percentage of baseline values) correlated positively with those in plasma NA (r= 0.598, p<0.001). Heart rate, and blood pressure The resting values of heart rate (HR), systolic (BPs) and diastolic (BPd) arterial blood pressures were within normal limits. The handgrip caused significant increases in HR, BPs and BPd (p<0.001) already at the 3rd min of exercise in all subjects. The values obtained at the 6th min were significantly higher than those at the 3rd minute of exercise (p<0.001). After 5 min recovery period, HR, BPs and BPd returned to the resting values (Fig.

1). Figure 1 Heart rate, systolic and diastolic blood pressure, peak velocity and mean acceleration of blood flow in the ascending aorta at rest, during handgrip (3�� and 6��) and at the 5th min of the recovery period (rec.). Values are means �� … Significant positive correlations were ascertained between the exercise-induced increases in BPs (expressed as percentage of baseline values) and those in plasma ET-1 (r= 0.697, p<0.001) as well as between the exercise-induced increases in BPd and those in plasma ADM (r= 0.789, p<0.001). Doppler echocardiographic indices of left ventricular systolic function The resting values of PV and MA were within normal limits. The static handgrip caused declines in PV (p<0.001) and MA (p<0.01) in all subjects.

The decreases in PV and MA during the second bout of exercise were significantly lower than those during the first bout (p<0.05). After 5 min recovery period, PV and MA did not differ significantly from the resting values (Fig. 1). Significant relationships were found between the exercise-induced decreases in both PV and MA (expressed as percentage of baseline values) and increases in plasma Entinostat ADM (r=?0.679, p<0.001 and r=?0.619, p<0.001; respectively) and ET-1 (r=?0.665, p<0.001 and r=?0.599, p<0.001; respectively; Fig. 3).