We thank Nortura SA for their support when obtaining lamb, pork and beef
samples. Professor Jan Gebicki is acknowledged for sharing his experience with the FOX-assay with us. This work was supported by project 224798 granted by FFL/JA. “
“Honey is characterised by its complex composition, which varies with the origin of the raw material as nectar or honeydew, PD0332991 concentration the bee species, the edaphoclimatic conditions, the available floral source and the storage conditions (Gheldof & Engeseth, 2002). Honey mainly consists of glucose and fructose but also contains amino acids, phenolic compounds, organic acids, vitamins, minerals, lipids, enzymes and other phytochemicals (Baltrušaityte, Venskutonis, & Čeksteryte, 2007). In the Northern and Northeastern regions of Brazil, there are several native stingless bee species that produce honey and are known as indigenous bees, stingless or meliponini (Silva et al., 2013). In the state of Amazonas, among the several species already identified, attention should be given to Melipona (Michmelia) seminigra merrillae Cockerell, 1919 (Hymenoptera: Apidae: Meliponini), which is endemic Fasudil clinical trial to Central Amazonia and is an important pollinator of the Amazon rainforest (e.g., Theobroma grandiflorum Schum,
Bixa orellana L., Euterpe oleracea Mart., and Paullinia cupana Kunth). The growing interest in the honey produced by stingless bees proceeds from its composition, which has been associated with antiseptic, antimicrobial, anticancer, anti-inflammatory, and wound-healing properties
and may provide defence for and promote cell functions in erythrocytes ( Alvarez-Suarez et al., 2012, Silva et al., 2006, Silva et al., 2013 and Vit and Tomás-Barberán, 1998). Hundreds of bioactive substances have already been found in honeys from the Melipona species in different countries ( Oddo et al., 2008, Oliveira et al., 2012 and Silva et al., 2013). Among the compounds with biological activity that are present Methisazone in honeys, the compounds that display antioxidant capacity, such as phenolic acids, flavonoids and the enzymes glucose oxidase and catalase, have received special attention from research groups, due to their role in the prevention of diseases associated with oxidative stress ( Aljadi & Kamaruddin, 2004). Silva et al. (2013) studied the phenolic profile of the ethyl acetate fraction of Melipona honey extract (M. subnitida) collected in Paraíba State and reported a strong relation between the results of the DPPH (1,1-diphenyl-2-picryl hydrazyl) and ABTS (2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid) antioxidant assays and the phenolic compounds 3,4-dihydroxybenzoic acid, gallic acid, and vanillic acid.