Factor Discriminant Analysis (FDA). FDA included in XLStat 7.5 software was performed to create a predictive model useful to classify the patients into one of the three groups according to their TTGE profile. Wilk’s Lambda test was used and a P value less than or equal to 0.05 was considered statistically significant. Partial Least Square
Discriminant Analysis (PLS-DA). PLS-DA included in SIMCA+ software (UMETRICS, Umea, Sweden) was performed to depict score plot of TTGE profiles by means of principal components PC1 and PC2, and to assess TTGE band importance. Data were automatically mean centred and unit variance (UV) scaled LY411575 nmr by the statistical software. Each TTGE band was hierarchically classified based on a find more software-assigned variable importance (VIP) value. The variables with VIP value > 1 were chosen as discriminatory. Non-parametric statistical methods. For Shannon-Weaver index, species-specific Defactinib PCR, FDA and PLS-DA, a bilateral Wilcoxon signed rank test was utilized to compare active and inactive CD patients’ groups, whilst a bilateral Mann-Whitney U-test was utilized to compare active/inactive CD patients with control group. A P value less than
or equal to 0.05 was considered statistically significant. Acknowledgements Grants: This work was supported by MIUR grants to SC and University grants to SS and MC. References 1. Farrell RJ, Kelly CP: Celiac sprue. N Engl J Med 2002, 346:180–188.PubMedCrossRef 2. Fortnightly FC: Coeliac disease. Br Med J 1999, 319:236–239. 3. Ciccocioppo R, Di Sabatino A, Corazza GR: The immune recognition of gluten in coeliac disease. check details Clin Exp Immunol 2005, 140:408–416.PubMedCrossRef
4. Qiao SW, Bergseng E, Molberg Ø, Jung G, Fleckenstein B, Sollid LM: Refining the rules of gliadin T cell epitope binding to the disease-associated DQ2 molecule in celiac disease: importance of proline spacing and glutamine deamidation. J Immunol 2005, 175:254–261.PubMed 5. Tjellstrom B, Stenhammar L, Hogberg L, Fälth-Magnusson K, Magnusson KE, Midtvedt T, Sundqvist T, Norin E: Gut microflora associated characteristics in children with celiac disease. Am J Gastroenterol 2005, 100:2784–2788.PubMedCrossRef 6. Nadal I, Donant E, Koninckx CR, Calabuig M, Sanz Y: Imbalance in the composition of the duodenal microbiota of children with coeliac disease. Journal of Medical Microbiology 2007, 56:1669–1674.PubMedCrossRef 7. Sanz Y, Sanchez E, Marzotto M, Calabuig M, Torriani S, Dellaglio F: Differences in faecal bacterial communities in coeliac and healthy childrens detected by PCR and denaturing gradient gel electrophoresis. FEMS Immunol Med Microbiol 2007, 51:562–568.PubMedCrossRef 8. Collado MC, Calabuig M, Sanz Y: Differences between the Faecal Microbiota of Coeliac Infants and Healthy Controls. Curr Issues Intestinal Microbiol 2007, 8:9–14. 9.